Box C/D snoRNA SNORD89 influences the occurrence and development of endometrial cancer through 2’-O-methylation modification of Bim

The small nucleolar RNA (snoRNA) is a type of small non-coding RNA widely distributed in the nucleoli of eukaryotic cells, promoting cancer development. The aim of this study was to assess box C/D snoRNA 89 (SNORD89) dysregulations in endometrial cancer. According to the TCGA database as well as the International Federation of Gynecology and Obstetrics (FIGO), higher SNORD89 expression is found in endometrial cancer tissues. In addition, the SNORD89 expression level was higher in endometrial carcinoma with lymph node metastasis than in endometrial carcinoma without lymph node metastasis. By interacting with the conservative chaperone protein methylase fibrillarin (Fbl), SNORD89 inhibits the translation process of the Bim gene, leading to a decrease in Bim protein. Cancer-promoting effect of SNORD89 can be reversed by Fbl knockdown or Bim overexpressing. What’s more, ASO-mediated silencing of SNORD89 could inhibit endometrial cancer cell proliferation and migration ability. Taken together, SNORD89 can modify Bim through 2′-O-methylation and affect downstream signaling pathways to promote endometrial cancer occurrence and development. The role of methylation modification in the prevention and treatment of endometrial cancer provides a new understanding and SNORD89 may be a new diagnostic and therapeutic target for endometrial cancer.


INTRODUCTION
Endometrial cancer is the most common gynecological malignant tumor in developed countries. Its morbidity and mortality also continue to soar [1]. The 5-year relative survival rate of early endometrial cancer is high (96%). However, late diagnosis reduces the 5-year relative survival rate dramatically (18%) [2]. Endometrial cancers with high malignancy and poor prognosis are characterized by the loss of tumor suppressor genes or amplification of multiple oncogenes. Therefore, an in-depth investigation of the molecular mechanism of the occurrence and development of endometrial cancer is essential for the early detection, diagnosis, and treatment of endometrial cancer. The small nucleolar RNA (snoRNA) is a non-coding RNA (ncRNA) [3,4]. At present, ncRNA is generally believed to potentially regulate various cellular processes, including chromosome remodeling, transcription, translation, and signal transduction [5].
The abnormal expression and dysfunction of ncRNA are also involved in the occurrence and development of multiple malignant tumors, such as liver cancer [6], endometrial carcinoma [7], and colorectal cancer [8]. snoRNA is a type of small ncRNA widely distributed in the nucleus of eukaryotic cells, with a length of 60-300 nt. It can bind to four nucleolar ribonucleoproteins (Fbl/ Nop1p, Nop56, Nop58/Nop5p, and P15.5 KD/Snu13p) to form a snoRNP complex and participate in ribosomal RNA (rRNA) processing, RNA shearing, and translation to process regulation and oxidative stress response [9]. Several recent studies have reported that snoRNAs are closely related to cancer occurrence and development. For example, snoRNA42 overexpression is related to the invasive biological behavior of non-small cell lung cancer (NSCLC), and its expression level is inversely proportional to the overall survival rate of patients, suggesting that snoRNA42 may become a new prognostic marker and potential therapeutic target of NSCLC [10]. In addition, snoRNA73A, snoRNA73B, and snoRNA74A can activate poly [ADP-ribose] polymerase (PARP-1) to regulate downstream signaling pathways and participate in breast cancer development by combining with the DNA repair enzyme PARP-1 in the absence of DNA damage stimulation [11]. Moreover, SNORD126 activates the PI3K-AKT pathway in liver and colorectal cancer by combining with hnRNPK to promote tumor cell proliferation in vitro and tumor formation in xenograft models in vivo [12]. Ravo et al. conducted high-throughput genetic analysis on paired normal, hyperplastic, and cancerous endometrial tissues obtained by endometrial biopsy. They found increased SNORD116 and SNORD2 expression, while SNORD3 was decreased in cancerous endometrial tissues [13]. This suggests that snoRNA is closely related to the occurrence and development of endometrial cancer. However, the specific mechanism of action and possible signaling pathways of snoRNA in endometrial cancer has not yet been reported.
SNORD89 belongs to the Box C/D snoRNAs in snoRNA, located at 2q11.2, also known as HBII-289. It is characterized by highly conserved boxes, called C (typical motif RUGAUGA, where R is purine) and D (typical motif CUGA). Box C/D snoRNAs could combine with Fbl/Nop1p, Nop56, Nop58/Nop5p, and P15.5 KD/ Snu13p to form a snoRNP complex and 2′-O-methylate modification of rRNA, transfer RNA (tRNA), and other RNAs, among which Fbl is the key 2′-O-methylase of Box C/D snoRNA [14]. Besides, studies have shown that some snoRNAs can be processed into miRNA precursors, forming snoRNA-like microRNAs (sno-miRNAs) similar to snoRNA that plays a miRNA-like role [15]. miRNA-like roles are the same functions played by miRNAs and can silence the expression of target genes. AGO2 is the core element of the RNA-induced silencing complex. It not only promotes the degradation of the target mRNA and inhibits its protein translation in the miRNA/siRNA pathway, but also regulates the biosynthesis and maturation of miRNA, playing a key role in the regulatory pathway [16][17][18].
According to the function, members of the Bcl-2 family can be divided into three groups: two groups of antiapoptotic members and one group of proapoptotic members. Bcl-2 members mostly contain Bcl-2 tumor domains (BH domains), ranging from BH1 to BH4. Bim belongs to the only BH3 proapoptotic group of the Bcl-2 family, promoting many tumors and cell disorders, such as lung cancer [19], breast cancer [20,21], osteosarcoma [22], prostatic cancer [23], and melanoma [24]. Various chemotherapeutic drugs contain Bim as an intermediary executor of cell death [25,26]. Bim is located in the cytoplasm and can induce apoptosis by directly activating the proapoptotic Bax/Bak or by neutralizing the antiapoptotic Bcl-2 protein (Bcl-2 and Bcl-XL) when activated by foreign signals. When activated directly or indirectly by Bim, Bax will be inserted into the mitochondrial outer membrane (MOM) and oligomerized with Bax/Bak into the pore, enhancing the permeability of MOM and promoting the release of mitochondrial interstitial membrane proteins such as Cytc into the cytoplasm. After Cytc enters the cytoplasm, it can bind to apoptotic protease activator 1 (Apaf-1) to activate Apaf-1 and its oligomer into a sevenfold caspase activation complex, known as Apaf-1 apoptotic body. Apaf-1 can successively recruit and activate caspase-9 and caspase-3 to participate in the hydrolysis of various proteins and finally induce cell apoptosis [27]. In addition to directly activating Bax and promoting apoptosis, Bim can exert an antiapoptotic effect by antagonizing the antiapoptotic protein Bcl-2, which can not only bind Bax directly to inhibit Bax activation but also bind the Apaf-1 apoptotic body to inhibit the apoptosis process.
In this study, to explore the possible molecular mechanism of SNORD89 in endometrial cancer we performed in vitro and in vivo experiments.

SNORD89 expression is increased in endometrial carcinoma
In the TCGA database and starbase database analysis, the expression of SNORD89 in endometrial cancer tissues was higher than that in normal endometrial tissues (Fig. 1A, B, *P < 0.05). qRT-PCR detected significantly increased expression of SNORD89 in endometrial cancer tissues compared with its expression in normal endometrial tissues (Fig. 1C, D, *P < 0.05). In the analysis of the relationship between SNORD89 expression level and clinicopathological features of endometrial cancer, the SNORD89 expression level was higher in the International Federation of Gynecology and Obstetrics (FIGO) stage II-IV diseases than in FIGO stage I diseases (Fig. 1E, *P < 0.05). In addition, the SNORD89 expression level was higher in endometrial carcinoma with lymph node metastasis than in endometrial carcinoma without lymph node metastasis (Fig. 1F, *P < 0.05). qRT-PCR analyses revealed that SNORD89 expression was the lowest in Ishikawa cells, but the highest in HEC-1B cells (Fig. 1G, *P < 0.05). Therefore, Ishikawa cells were used for transfection of SNORD89 overexpression plasmid, and HEC-1B cells were used for SNORD89 ASO transfection. qRT-PCR was used to confirm the transfection efficiency of plasmid-or ASO-transfected cells ( Fig. 2A, E, *P < 0.05). In vivo effect of SNORD89 overexpression Following the experiments detailed in the Materials and methods section, a subcutaneous tumor formation experiment was performed in nude mice to detect the in vivo effect of SNORD89. The results showed that compared with the control group, the tumor formation rate was faster, and the tumor volume was larger in mice injected with SNORD89 Ishikawa-overexpressed cells ( Fig. 3A-D, *P < 0.05).

SNORD89 downregulates Bim gene expression via Fbl
A possible binding site between the tumor suppressor gene Bim and SNORD89 was found through the gene complementary pairing with SNORD89 sequence base by bioinformatics prediction (NCBI database) (Fig. 4A). RIP experiments confirmed that Bim could combine with SNORD89, and the expression level of Bim protein decreased after SNORD89 was overexpressed in Ishikawa cells (Fig. 4B, C, *P < 0.05). Meanwhile, we found that overexpression of Bim in SNORD89-overexpressed Ishikawa cells reverses the cell survival effect of SNORD89. (Fig. 4E, F, *P < 0.05). Therefore, it was hypothesized that SNORD89 might play an oncogenic role in endometrial cancer by regulating Bim. SNORD89 performs 2′-O-methylation modification mainly by binding to RNA-methylated proteins such as Fibrillarin (Fbl) or competitive endogenous RNA by binding to proteins such as Argonaute (AGO2). RIP experimental results showed that SNORD89 could not bind AGO2 but could bind the Fbl protein (Fig. 4B, *P < 0.05). To further verify that SNORD89 regulates Bim expression through Fbl, we knocked down Fbl in Ishikawa cells with overexpressed SNORD89, Fbl knockdown reversed the antiapoptotic effect of SNORD89 (Fig. 4G, H, *P < 0.05), suggesting that in combination with Fbl SNORD89 suppresses Bim gene expression.
The relationship between SNORD89 and Bim 2′-O-methylation was detected by the RTL-P experiment In order to verify whether SNORD89 combined with FBL regulates Bim expression by affecting the 2′-O-methylation modification level, we used the RTL-P experiment for detection, and the specific experimental method was obtained from existing literature. Figure  5A illustrates the principle of the RTL-P experiment, in which reverse transcriptase immediately stops cDNA synthesis before encountering 2′-O-methylated nucleotides at low concentrations of dNTPs. RTL-P experimental results showed that the Bim products of SNORD89 overexpressed were significantly reduced at low concentration, suggesting higher 2′-O-methylation activity and more 2′-O-methylation sites (Fig. 5B, *P < 0.05).
Relationship between SNORD89 overexpression and Bim mRNA stability We performed RNA stability tests in SNORD89-overexpressed Ishikawa cells, and the results confirmed that SNORD89 overexpression did not affect the stability of Bim mRNA (Fig. 5C, *P < 0.05). The above results indicated that SNORD89 combined with Fbl increased the level of 2′-O-methylation modification of Bim mRNA, affecting the complementary base pairing of Bim mRNA, thus reducing Bim protein.
Changes in protein levels in endometrial cancer cell lines after SNORD89 overexpression The above results indicated that SNORD89 regulated Bim 2′-Omethylation modification by binding Fbl to form a snoRNP complex, thus reducing the Bim protein level. Through the KEGG database (Fig. 5D), we found that Bim/Bcl-2/Bax was an important signaling pathway that mediated apoptosis. Western blotting showed that after SNORD89 overexpression, the Bcl-2 protein level and Bcl-2/Bax ratio increased (Fig. 5E, *P < 0.05). The results of the rescue experiment revealed that Bcl-2 protein level and Bcl-2/Bax ratio were decreased after Fbl silencing in Ishikawa cells with overexpressed SNORD89 (Fig. 5F, *P < 0.05). The above results indicated that SNORD89 was involved in the occurrence and development of endometrial cancer by combining Fbl to form a snoRNP complex and regulating the Bim/Bcl-2/Bax signaling pathway.
SNORD89 overexpression can decrease Bim protein level and increase Bcl-2 protein level in vivo Immunohistochemical analysis of tumor tissues collected in vivo from subcutaneous tumorigenesis experiment in nude mice showed decreased expression of the Bim protein and increased expression of the Bcl-2 protein in tumor tissues generated by Ishikawa cells with overexpressed SNORD89 (Fig. 6A). By extracting tumor tissue proteins and conducting Western blotting experiments, the Bcl-2 protein increased and Bim protein levels decreased in tumor tissues with overexpressed SNORD89 (Fig.  6B, C, *P < 0.05). The above results further verified that SNORD89 was involved in the occurrence and development of endometrial cancer by regulating the Bim/Bcl-2/Bax signaling pathway.

DISCUSSION
To search for diagnostic and therapeutic markers of endometrial cancer, we screened various snoRNAs from the TCGA database and found a significantly increased expression of SNORD89 in endometrial cancer tissues compared with normal endometrial tissues. We further examined the expression of SNORD89 in our clinical samples, the result of which was still the case. We speculated that it was related to the FIGO stage and lymph node metastasis and that SNORD89 might be involved in the occurrence and progression of endometrial cancer and affect the prognosis.
To evaluate the function of SNORD89 in endometrial cancer cells, we performed in vitro cell experiments and in vivo subcutaneous tumorigenesis experiments in nude mice. The transfection of SNORD89-overexpressing plasmids into Ishikawa cells with low SNORD89 expression promoted cell proliferation, inhibited cell apoptosis, and induced cell migration. However, the cell experiment results were reversed after the expression level of SNORD89 was knocked down in HEC-1B cells. In vivo subcutaneous tumorigenesis experiments in nude mice also confirmed that SNORD89 overexpression can induce tumor growth in vivo. These results indicated that SNORD89 had an oncogenic effect in endometrial cancer. However, the specific mechanism of action of SNORD89 in endometrial cancer remained unclear.
We proved through the RIP experiment that SNORD89 does not combine with AGO2, but with Fbl. Fbl silencing inhibited the proliferation of ovarian cancer cells with stable overexpression of SNORD89. Therefore, we believe that SNORD89 may promote the development and progression of endometrial cancer by influencing the 2′-O-methylation modification of target genes.
BLAST search for downstream genes complementary to the SNORD89 sequence revealed a binding point might exist between SNORD89 and Bim. The RIP experiment confirmed that Fbl could bind with Bim and that SNORD89 overexpression significantly reduced the expression of Bim protein. Therefore, we believe that SNORD89 may be involved in the progression of endometrial cancer by regulating Bim. In order to verify that SNORD89 plays its carcinogenic role through 2′-O-methylation modification of Bim mRNA, we designed corresponding primers and detected whether there was 2′-Omethylation modification at SNORD89 and Bim pairing site by the RTL-P method. The results confirmed that Bim methylation modification was enhanced after overexpression of SNORD89.
2′-O-methylation modification refers to the addition of methyl at the 2′ positions of nucleic acid ribose so that the 2′-OH group is replaced by 2′-O-methyl [28]. This modification is mainly mediated by snoRNAs and has been confirmed to be involved in various cancers. For example, long ncRNA ZFAS1 promotes snoRNAmediated 2′-O-methylation through Nop58 recruitment and is involved in the development and prognosis of colorectal cancer [29]. Methyltransferase HENMT1 inhibits apoptosis of lung cancer cells by enhancing its stability by 2′-O-methylation of the 3′terminal of Mir-21-5p [30]. Several studies have demonstrated that 2′-O-methylation modification was mainly located in rRNA [31]. Recently, some studies have shown that snoRNA could perform 2′-O-methylation modification on the mRNA of the target gene, and these modification sites could regulate mRNA and protein expression of the target gene [32].
2′-O-methylation modification can protect mRNA from hydrolysis, thus increasing the mRNA stability. However, this modification limits RNA chain conformation and suppleness, interferes with the ribosome monitoring base on the space and the homologous codon-anticodon spiral interaction, causes the excessiveness of homologous aminoacylation tRNAs in the initial selection and calibration to refuse, and inhibits the expression of target genes. Further, through RNA stability and Western blotting experiments, we confirmed that SNORD89 do not alter the stability of Bim mRNA, but reduces the expression level of Bim protein. This suggests that SNORD89 may promote the occurrence and development of endometrial cancer by binding Fbl to form a snoRNP complex 2′-O-methylation to modify Bim mRNA and reduce the expression level of Bim protein.
Bim is a member of the Bcl-2 family. Bcl-2 family proteins constitute key intracellular checkpoints and regulators of cell death and can inhibit or promote apoptosis. Irregularities in the members of the Bcl-2 family are found in various cancers and diseases, including breast cancer [33] and prostate cancer [34]. Some studies have suggested that the intracellular Bcl-2/Bax protein ratio is the key to cell survival [35]. We found that the Bcl-2/Bax protein ratio increased significantly after SNORD89 overexpression. Suggesting that SNORD89 may affect downstream signaling pathways through Bim. Meanwhile, the rescue experiment showed that Bim protein increased after Fbl knockdown in the Ishikawa cell line stably transfected with overexpressed SNORD89 plasmid. These results suggest that Fbl protein plays an important role in SNORD89's regulation of Bim. Therefore, we believe that SNORD89 may modify Bim mRNA through 2′-Omethylation, reduce the expression level of Bim protein, increase the ratio of Bcl2 to Bax, and inhibit the activation of Bax, the release of mitochondrial Cytc, and subsequent apoptosis.
Finally, immunohistochemical analysis of tumor tissues collected from subcutaneous tumorigenesis experiment in nude mice confirmed that SNORD89 overexpression in vivo could decrease the expression of Bim protein and increase the expression of Bcl-2 protein in endometrial cancer cells. By extracting tumor tissue proteins and Western blotting experiments, the Bcl-2 protein were increased, and the levels of Bim proteins were decreased in tumor tissues overexpressing SNORD89. SNORD89 can be further verified by influencing Bim and its mediated Bcl-2/Bax signaling axis to participate in the occurrence and development of endometrial cancer.

CONCLUSIONS
SNORD89 can modify Bim through 2′-O-methylation and affect downstream signaling pathways to promote endometrial cancer occurrence and development. It enables snoRNA-mediated RNA 2′-O-methylation. The role of methylation modification in the prevention and treatment of endometrial cancer provides a new understanding and SNORD89 may be a new diagnostic and therapeutic target for endometrial cancer.

Cell proliferation was detected by cell counting kit 8 (CCK8)
Cells were inoculated at 3000/well in 96-well plates, and transient transfection was performed after cell adherence. At specific time points (0, 24, 48, and 72 h), 10 μL of Cell Counting Kit 8 (CCK8, Yeasen, Shanghai, China) was added to each medium and cultured at 37°C for 2 h. Then the absorbance was read at 450 nm. The experiment was repeated three times.

Wound-healing assay
In this experiment, 6 × 10 5 cells were seeded in 6-well plates. A vertical wound was marked in the Petri dish with the tip of a 200-μL pipette. Cells were cleaned three times with phosphate-buffered saline (PBS), and a 2-mL serum-free medium was added to the Petri dish. Wound healing was recorded at 0, 24, and 48 h. Image J software (National Institutes of Health, Bethesda, MD, USA) was used to determine the wound size and calculate the cell migration rate.

Flow cytometry analysis of apoptosis
Apoptosis was detected by Annexin V-fluorescein isothiocyanate (FITC)/ propidium iodide (PI) double staining according to the manufacturer's protocol. Cells 3 × 10 5 /well were inoculated in six-well plates and cultured to a cell density of 60-70% for transient transfection. At 48 h after transfection, the cells were washed with cold PBS twice, digested, suspended with trypsin without ethylenediaminetetraacetic acid (1 × 10 6 cells/mL), and cleansed with cold PBS three times. Cells were then cultured in 100 µL of binding buffer containing 5 µL of Annexin V-FITC and 5 µL of PI for 15 min in 4°C darkness, with 400 µL of the binding buffer added to each tube. The cells were quantitatively analyzed by flow cytometry.

Real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR)
An RNA Quick Purification kit was used to extract the total RNA from cells and tissues, and a Fast Reverse Transcription kit was used to generate endogenous complementary DNA. The cDNA was used as a template for qRT-PCR using the SYBR Prime X Ex-TAQ Patent II Suite (Takara Bio Inc., Shiga, Japan). Finally, the relative expression of the gene was calculated by comparing the period threshold (Ct) value of the target gene with the control gene (U6, encoding glyceraldehyde-3-phosphate dehydrogenase or 18sRNA gene) according to the 2−ΔCt method.

RNA-binding protein immunoprecipitation (RIP) detection
TRIzol reagent (1 mL; Takamura, Shibuya, Japan) was used to extract the total RNA from cells or tissues. Then, 200 μL of chloroform was added to the extract, fully mixed, rested for 5 min, and centrifuged at 12,000 rpm for 20 min. The upper clarifying liquid was transferred to a new tube, and the RNA was precipitated with the same amounts of isopropyl alcohol. After centrifugation at 12,000 rpm for 20 min, the precipitate was retained and cleaned with 75% ethanol. Once fully dried at room temperature, sodium diethylpyrocarbonate (DEPC) water was used to dissolve the residue. The total RNA concentration was determined by spectrophotometry (Thermo Fisher Scientific, Shanghai, China). Then, using reverse transcriptase and random primers, 2 μg of RNA was reverse-transcribed into complementary Values are presented as the mean ± SD. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. DNA (cDNA) according to the manufacturer's protocol (Yeasen). The cDNA was used as a template for qRT-PCR using the SYBR Prime X Ex-TAQ Patent II Suite (Takara Bio Inc., Shiga, Japan). Finally, the relative expression of the gene was calculated by comparing the period threshold (Ct) value of the target gene with the control gene (U6, encoding glyceraldehyde-3phosphate dehydrogenase or 18sRNA gene) according to the 2−ΔCt method.

Subcutaneous tumorigenesis experiment in nude mice
The detection kit for subcutaneous tumor transmission was provided by Guangdong Medical Laboratory Animal Center (Guangdong, China) for BALB/C female nude mice, which were placed in a specific pathogen-free environment. The nude mice were randomly divided into groups according to the label. Ishikawa cells transfected with SNORD89/no-load plasmid (1 × 10 7 in 150 μL of FBS-free medium) were subcutaneously injected into 5-week-old female mice to establish a tumor-forming nude mice model. After tumor formation, the tumor volume was measured weekly with vernier calipers to understand tumor growth. When the tumor reached a certain size, the mice were euthanized, the tumor nodules were resected, and their volume was measured. No blinding was done.

Immunohistochemistry
The tumor tissue was collected by treating it with xylene and 100% ethanol and then decreasing the ethanol concentration. After antigen retrieval, endogenous peroxidase activity was blocked by incubation in 3% hydrogen peroxide for 40 min, then the slides were washed three times with PBS, and incubated in 5% bovine serum albumin for 40 min, stained with anti-BIM antibody (1:100, Proteintech, USA) and BCL-2 antibody (1:100, Proteintech, USA), followed by enzyme-conjugated secondary antibody (Dako) incubation, washed in PBS for 5 min, developed using DAB reagent. Hematoxylin is used for redyeing and images taken with light microscopes are gained.

Reverse transcription at low dNTPs followed by PCR (RTL-P)
To detect the 2′-O-methylation (Nm) of SNORD89, RT-PCR was performed [32,37]. The total reaction system was 20 μl. Containing 5 μg total RNA, a low (1 μM) or high (1 mM) concentration dNTPs (TaKaRa) and 1 μl specific RT primers (PF) were denatured at 65°C for 5 min and then placed on ice. According to the instruction, 4 μl M-MLV RT 5×buffer (PROMEGA), 1 μl 200 u/μl M-MLV Reverse Transcriptase (PROMEGA), 1 μl 0.1 M DTT (PROMEGA), and 1 μl 40 u/μl RNase inhibitor (PROMEGA) were mixed at 50°C for 1 h, and then heated at 70°C for 15 min. cDNA is used as a template for QRT-PCR using the SYBR Prime X Ex-TAQ Patent II Suite (Takara). Finally, the relative expression of the gene was calculated by comparing the period threshold (Ct) of the target gene in the experimental group and the control group at low concentrations according to the 2−ΔCt method.

Ethical statement
The animal study was reviewed and approved by Guangdong Provincial Medical Experimental Animal Center (Approval no. S2021-101), with a license to use experimental animals [SYXK(Guangdong)2018-0002]. Written informed consent was obtained from the owners for the participation of their animals in this study.

Data analysis
GraphPad Prism (version 8.02(263)) was used for graphics and data analysis. Relevant data were expressed as mean ± standard mean error. Statistical analysis was performed using two-sample t-test and P < 0.05 was considered to indicate a significant difference.

DATA AVAILABILITY
The data generated or analyzed during this study are included in this published article and its supplementary information files, Supplementary information is available at Cell Death Discovery's website.